The Pánfilo de Narváez expedition passed to the west of Potano territory in 1528. While not engaging with the Potano, the Spanish incursion spread new infectious diseases and incited warfare by competing tribes in the area. In 1539 Hernando de Soto led an army through Potano territory. There were 700 or more people in de Sotò's army. They forced villagers to give upstored food to them. By the time de Sotò's army reached Potano territory, he was intent on spending the winter in the Apalachee domain, and the army passed through quickly. The army passed through Potano towns that the Spanish called Itaraholata , Potano, Utinamochana , Mala-paz and Cholupa. At the time the French established Fort Caroline, the Potano were at war with the Utina, a chiefdom ruled by Chief Utina or Outina. The French supported the Utina and helped defeat the Potano. After Spain expelled France from Florida, it also supported the Utina. In 1584 the Potano killed a Spanish captain leading an invasion into Potano territory. To punish them, a second Spanish expedition attacked and killed many Potano and drove the rest from their towns. After that attack, the town of Potano was moved to the FoxPond site near the Devil's Millhopper northwest of Gainesville. In the 1580s Spanish Franciscanmissionaries reached the Potano, first with visits by an itinerant missionary. A visita named Apula was established in the town of Potano, but was destroyed in the Spanish raid of 1584 or 1585. A couple of visitas existed in Potano territory in the 1590s. In 1606 Spanish missionaries established a doctrina, San Francisco de Potano, in the relocated town of Potano. This was the first doctrina west of the St. Johns River. Another doctrina, San Miguel de Potano, and a visita, Santa Ana de Potano, were soon established within a few miles of San Francisco de Potano. Another visita, San Buenaventura de Potano, was established at the former site of the town of Potano in 1607 or 1608 by Fray Francisco Pareja. The missionaries reported that they had baptized more than 1,000 adult Potanos by 1607. The missions of San Miguel and San Buenaventura disappeared from Spanish records within a few years. In 1656 the Potano participated in the Timucuan rebellion against the Spanish authorities. The Spanish prevailed after eightmonths. During the fighting, they had burned most of the Timucuan towns and missions. After the rebellion, the Spanish re-established the Potano missions. In 1672 the Potano suffered many deaths from an unidentified disease. One colonial estimate figured the population of the Potano at 3,000 in 1650. By 1675, only 160 people were surviving at the two Potano missions. Following the Timucuan rebellion, the Spanish made many land grants to their colonists in areas no longer used by the reduced Potano population. There is evidence of twenty-five Spanish-owned cattle ranches in Timucuan territory, including several in Potano territory. Much of the evidence for these ranches was recorded as Potano complaints to Spanish officials that cattle were running loose and eating village crops. By 1685, tribes from north of Florida, supplied with arms and often joined by English settlers from the Province of Carolina, were raiding Potano territory, burning villages, killing some Potano and carrying others away to be sold as slaves in Charles Towne. These raids continued into the 18th century. The Spanish mission system across northern Florida collapsed after 1704. Some surviving Potano may have reached the mission at St. Augustine as refugees, but the Potano effectively became extinct as a tribe at that time.
