Tryptic soy broth
Tryptic soy broth or Trypticase soy broth is used in microbiology laboratories as a culture broth to grow aerobic bacteria. It is a complex, general purpose medium that is routinely used to grow certain pathogenic bacteria, which tend to have high nutritional requirements. Its agar counterpart is tryptic soy agar. One of the components of Tryptic soy broth is , which is an enzymatic digest of soybean meal.
TSB is frequently used in commercial diagnostics in conjunction with the additive sodium thioglycolate which promotes growth of anaerobes.Preparation
To prepare TSB, the following ingredients are dissolved under gentle heat and then autoclaved for 15 minutes at.
