In 2003, Desai was a social worker with Krantiveer Jhopdi Vikas Sangh to help rehabilitate slum dwellers. From 2007 to 2009, Desai staged protests against the financial irregularities at the Ajit Cooperative Bank involving a fraud of. In January 2009, she led a group against then deputy Chief Minister of MaharashtraAjit Pawar. An arrest warrant was issued against her in 2013 for heading the group that allegedly "slapped the effigy of Pawar, used abusive language and held an illegal agitation despite prohibitory orders". Desai was immediately released on bail, claiming that the arrest was just a tactic to stop activists from protesting. She founded the Bhumata Brigade on 27 September 2010. Since its founding, the brigade has grown from 400 to 5,000 registered members as of 2016. In 2011, she also participated in the anti-corruption movement. She contested the 2012 elections for the Pune Municipal Corporation from Balaji Nagar ward as a member of Indian National Congress; but lost.
Religious places
In November 2015, a woman entered the Hindu shrine of Shani Shingnapur Temple, where women were not allowed. The priests of the temple suspended the security guard on duty at the time and carried out a cleansing ceremony of the idol. This event of discrimination towards women agitated Desai, who along with other members of her Brigade staged various forced entries into the shrine. The state Government and the district level court of Pune directed the temple officials to allow women in the shrine based on their constitutional rights. On 8 April 2016, the day celebrated as Gudi Padwa—the new year day of the Maharashtrian calendar—Desai along with other female members of the Brigade entered the shrine of Shani Shingnapur Temple. Post Shingnapur entry, Desai reached to the Mahalakshmi Temple in Kolhapur where the temple management committee allowed her entry but the priests became violent against her. Five priests were arrested for attacking Desai and the protestors. She also entered the inner sanctum of the Trimbakeshwar Shiva Temple near Nashik where she was peacefully escorted by the police, but only with wet clothes similar to how the temple allows men. In April 2016, she made an attempt to enter the Haji Ali Dargah in Mumbai; however, an angry mob made it unsuccessful. Desai claimed that she received a death threat if she again tried to enter the dargah—a type of Islamic shrine. On 12 May 2016, she made a second attempt and entered the mosque under tight security but not in the inner sanctum where women are not allowed. In November 2018, she made an unsuccessful attempt to visit the Sabarimala temple in Kerala during the Mandalam-Makaravilakk pilgrim season. The temple had legal as well as religious restrictions that prevented the entry of women of menstruating age since 1991 which was overturned through a historic verdict by the Supreme Court of India in October 2018. Even though a dozen women of menstruating age group had attempted to visit the temple after the verdict came, all of them proved unsuccessful largely due to protests. Trupti was also blocked by protesters at Cochin International Airport on 16 November 2018, while on her journey to Sabarimala. She decided to return after getting stranded inside the airport for more than 14 hours and vowed to come back again.
