Student's t-test


The t-test is any statistical hypothesis test in which the test statistic follows a Student's t-distribution under the null hypothesis.
A t-test is most commonly applied when the test statistic would follow a normal distribution if the value of a scaling term in the test statistic were known. When the scaling term is unknown and is replaced by an estimate based on the data, the test statistics follow a Student's t distribution. The t-test can be used, for example, to determine if the means of two sets of data are significantly different from each other.

History

The term "t-statistic" is abbreviated from "hypothesis test statistic". In statistics, the t-distribution was first derived as a posterior distribution in 1876 by Helmert and Lüroth. The t-distribution also appeared in a more general form as Pearson Type IV distribution in Karl Pearson's 1895 paper. However, the T-Distribution, also known as Student's T Distribution gets its name from William Sealy Gosset who first published it in English literature in his 1908 paper titled Biometrika using his pseudonym "Student" because his employer preferred staff to use pen names when publishing scientific papers instead of their real name, so he used the name "Student" to hide his identity. Gosset worked at the Guinness Brewery in Dublin, Ireland, and was interested in the problems of small samples – for example, the chemical properties of barley with small sample sizes. Hence a second version of the etymology of the term Student is that Guinness did not want their competitors to know that they were using the t-test to determine the quality of raw material. Although it was William Gosset after whom the term "Student" is penned, it was actually through the work of Ronald Fisher that the distribution became well known as "Student's distribution" and "Student's t-test".
Gosset had been hired owing to Claude Guinness's policy of recruiting the best graduates from Oxford and Cambridge to apply biochemistry and statistics to Guinness's industrial processes. Gosset devised the t-test as an economical way to monitor the quality of stout. The t-test work was submitted to and accepted in the journal Biometrika and published in 1908. Company policy at Guinness forbade its chemists from publishing their findings, so Gosset published his statistical work under the pseudonym "Student".
Guinness had a policy of allowing technical staff leave for study, which Gosset used during the first two terms of the 1906–1907 academic year in Professor Karl Pearson's Biometric Laboratory at University College London. Gosset's identity was then known to fellow statisticians and to editor-in-chief Karl Pearson.

Uses

Among the most frequently used t-tests are:
Most test statistics have the form, where and are functions of the data.
may be sensitive to the alternative hypothesis, whereas is a scaling parameter that allows the distribution of to be determined.
As an example, in the one-sample t-test
where is the sample mean from a sample, of size, is the standard error of the mean, is the estimate of the standard deviation of the population, and is the population mean.
The assumptions underlying a t-test in its simplest form are that
In the t-test comparing the means of two independent samples, the following assumptions should be met:
Most two-sample t-tests are robust to all but large deviations from the assumptions.
For exactness, the t-test and Z-test require normality of the sample means, and the t-test additionally requires that the sample variance follows a scaled χ distribution, and that the sample mean and sample variance be statistically independent. Normality of the individual data values is not required if these conditions are met. By the central limit theorem, sample means of moderately large samples are often well-approximated by a normal distribution even if the data are not normally distributed. For non-normal data, the distribution of the sample variance may deviate substantially from a χ distribution. However, if the sample size is large, Slutsky's theorem implies that the distribution of the sample variance has little effect on the distribution of the test statistic.

Unpaired and paired two-sample ''t''-tests

Two-sample t-tests for a difference in mean involve independent samples or paired samples. Paired t-tests are a form of blocking, and have greater power than unpaired tests when the paired units are similar with respect to "noise factors" that are independent of membership in the two groups being compared. In a different context, paired t-tests can be used to reduce the effects of confounding factors in an observational study.

Independent (unpaired) samples

The independent samples t-test is used when two separate sets of independent and identically distributed samples are obtained, one from each of the two populations being compared. For example, suppose we are evaluating the effect of a medical treatment, and we enroll 100 subjects into our study, then randomly assign 50 subjects to the treatment group and 50 subjects to the control group. In this case, we have two independent samples and would use the unpaired form of the t-test.

Paired samples

Paired samples t-tests typically consist of a sample of matched pairs of similar units, or one group of units that has been tested twice.
A typical example of the repeated measures t-test would be where subjects are tested prior to a treatment, say for high blood pressure, and the same subjects are tested again after treatment with a blood-pressure-lowering medication. By comparing the same patient's numbers before and after treatment, we are effectively using each patient as their own control. That way the correct rejection of the null hypothesis can become much more likely, with statistical power increasing simply because the random interpatient variation has now been eliminated. However, an increase of statistical power comes at a price: more tests are required, each subject having to be tested twice. Because half of the sample now depends on the other half, the paired version of Student's t-test has only degrees of freedom. Pairs become individual test units, and the sample has to be doubled to achieve the same number of degrees of freedom. Normally, there are degrees of freedom.
A paired samples t-test based on a "matched-pairs sample" results from an unpaired sample that is subsequently used to form a paired sample, by using additional variables that were measured along with the variable of interest. The matching is carried out by identifying pairs of values consisting of one observation from each of the two samples, where the pair is similar in terms of other measured variables. This approach is sometimes used in observational studies to reduce or eliminate the effects of confounding factors.
Paired samples t-tests are often referred to as "dependent samples t-tests".

Calculations

Explicit expressions that can be used to carry out various t-tests are given below. In each case, the formula for a test statistic that either exactly follows or closely approximates a t-distribution under the null hypothesis is given. Also, the appropriate degrees of freedom are given in each case. Each of these statistics can be used to carry out either a one-tailed or two-tailed test.
Once the t value and degrees of freedom are determined, a p-value can be found using a table of values from Student's t-distribution. If the calculated p-value is below the threshold chosen for statistical significance, then the null hypothesis is rejected in favor of the alternative hypothesis.

One-sample ''t''-test

In testing the null hypothesis that the population mean is equal to a specified value, one uses the statistic
where is the sample mean, is the sample standard deviation and is the sample size. The degrees of freedom used in this test are.
Although the parent population does not need to be normally distributed, the distribution of the population of sample means is assumed to be normal.
By the central limit theorem, if the observations are independent and the second moment exists, then will be approximately normal N.

Slope of a regression line

Suppose one is fitting the model
where is known, and are unknown, is a normally distributed random variable with mean 0 and unknown variance, and is the outcome of interest. We want to test the null hypothesis that the slope is equal to some specified value .
Let
Then
has a t-distribution with degrees of freedom if the null hypothesis is true. The standard error of the slope coefficient:
can be written in terms of the residuals. Let
Then score is given by:
Another way to determine the score is:
where r is the Pearson correlation coefficient.
The score, intercept can be determined from the score, slope:
where is the sample variance.

Independent two-sample ''t''-test

Equal sample sizes and variance

Given two groups, this test is only applicable when:
Violations of these assumptions are discussed below.
The statistic to test whether the means are different can be calculated as follows:
where
Here is the pooled standard deviation for and and are the unbiased estimators of the variances of the two samples. The denominator of is the standard error of the difference between two means.
For significance testing, the degrees of freedom for this test is where is the number of participants in each group.

Equal or unequal sample sizes, similar variances ( \frac{1}{2} < \frac{s_{X_1}}{s_{X_2}} < 2)

This test is used only when it can be assumed that the two distributions have the same variance.
The previous formulae are a special case of the formulae below, one recovers them when both samples are equal in size:.
The statistic to test whether the means are different can be calculated as follows:
where
is an estimator of the pooled standard deviation of the two samples: it is defined in this way so that its square is an unbiased estimator of the common variance whether or not the population means are the same. In these formulae, is the number of degrees of freedom for each group, and the total sample size minus two is the total number of degrees of freedom, which is used in significance testing.

Equal or unequal sample sizes, unequal variances ( s_{X_1} > 2s_{X_2} or s_{X_2} > 2s_{X_1})

This test, also known as Welch's t-test, is used only when the two population variances are not assumed to be equal and hence must be estimated separately. The statistic to test whether the population means are different is calculated as:
where
Here is the unbiased estimator of the variance of each of the two samples with = number of participants in group . In this case is not a pooled variance. For use in significance testing, the distribution of the test statistic is approximated as an ordinary Student's t-distribution with the degrees of freedom calculated using
This is known as the Welch–Satterthwaite equation. The true distribution of the test statistic actually depends on the two unknown population variances.

Dependent ''t''-test for paired samples

This test is used when the samples are dependent; that is, when there is only one sample that has been tested twice or when there are two samples that have been matched or "paired". This is an example of a paired difference test. The t statistic is calculated as
where and are the average and standard deviation of the differences between all pairs. The pairs are e.g. either one person's pre-test and post-test scores or between-pairs of persons matched into meaningful groups. The constant is zero if we want to test whether the average of the difference is significantly different. The degree of freedom used is, where represents the number of pairs.

Worked examples

Let denote a set obtained by drawing a random sample of six measurements:
and let denote a second set obtained similarly:
These could be, for example, the weights of screws that were chosen out of a bucket.
We will carry out tests of the null hypothesis that the means of the populations from which the two samples were taken are equal.
The difference between the two sample means, each denoted by, which appears in the numerator for all the two-sample testing approaches discussed above, is
The sample standard deviations for the two samples are approximately 0.05 and 0.11, respectively. For such small samples, a test of equality between the two population variances would not be very powerful. Since the sample sizes are equal, the two forms of the two-sample t-test will perform similarly in this example.

Unequal variances

If the approach for unequal variances is followed, the results are
and the degrees of freedom
The test statistic is approximately 1.959, which gives a two-tailed test p-value of 0.09077.

Equal variances

If the approach for equal variances is followed, the results are
and the degrees of freedom
The test statistic is approximately equal to 1.959, which gives a two-tailed p-value of 0.07857.

Related statistical tests

Alternatives to the ''t''-test for location problems

The t-test provides an exact test for the equality of the means of two i.i.d. normal populations with unknown, but equal, variances. For moderately large samples and a one tailed test, the t-test is relatively robust to moderate violations of the normality assumption. In large enough samples, the t-test asymptotically approaches the z-test, and becomes robust even to large deviations from normality.
If the data are substantially non-normal and the sample size is small, the t-test can give misleading results. See Location test for Gaussian scale mixture distributions for some theory related to one particular family of non-normal distributions.
When the normality assumption does not hold, a non-parametric alternative to the t-test may have better statistical power. However, when data are non-normal with differing variances between groups, a t-test may have better type-1 error control than some non-parametric alternatives. Furthermore, non-parametric methods, such as the Mann-Whitney U test discussed below, typically do not test for a difference of means, so should be used carefully if a difference of means is of primary scientific interest. For example, Mann-Whitney U test will keep the type 1 error at the desired level alpha if both groups have the same distribution. It will also have power in detecting an alternative by which group B has the same distribution as A but after some shift by a constant. However, there could be cases where group A and B will have different distributions but with the same means. In such cases, MW could have more than alpha level power in rejecting the Null hypothesis but attributing the interpretation of difference in means to such a result would be incorrect.
In the presence of an outlier, the t-test is not robust. For example, for two independent samples when the data distributions are asymmetric or the distributions have large tails, then the Wilcoxon rank-sum test can have three to four times higher power than the t-test. The nonparametric counterpart to the paired samples t-test is the Wilcoxon signed-rank test for paired samples. For a discussion on choosing between the t-test and nonparametric alternatives, see Lumley, et al..
One-way analysis of variance generalizes the two-sample t-test when the data belong to more than two groups.

A design which includes both paired observations and independent observations

When both paired observations and independent observations are present in the two sample design, assuming data are missing completely at random, the paired observations or independent observations may be discarded in order to proceed with the standard tests above. Alternatively making use of all of the available data, assuming normality and MCAR, the generalized partially overlapping samples t-test could be used.

Multivariate testing

A generalization of Student's t statistic, called Hotelling's t-squared statistic, allows for the testing of hypotheses on multiple measures within the same sample. For instance, a researcher might submit a number of subjects to a personality test consisting of multiple personality scales. Because measures of this type are usually positively correlated, it is not advisable to conduct separate univariate t-tests to test hypotheses, as these would neglect the covariance among measures and inflate the chance of falsely rejecting at least one hypothesis. In this case a single multivariate test is preferable for hypothesis testing. Fisher's Method for combining multiple tests with alpha reduced for positive correlation among tests is one. Another is Hotelling's T statistic follows a T distribution. However, in practice the distribution is rarely used, since tabulated values for T are hard to find. Usually, T is converted instead to an F statistic.
For a one-sample multivariate test, the hypothesis is that the mean vector is equal to a given vector. The test statistic is Hotelling's t:
where is the sample size, is the vector of column means and is an sample covariance matrix.
For a two-sample multivariate test, the hypothesis is that the mean vectors of two samples are equal. The test statistic is Hotelling's two-sample t:

Software implementations

Many spreadsheet programs and statistics packages, such as QtiPlot, LibreOffice Calc, Microsoft Excel, SAS, SPSS, Stata, DAP, gretl, R, Python, PSPP, Matlab and Minitab, include implementations of Student's t-test.
Language/ProgramFunctionNotes
Microsoft Excel pre 2010TTESTSee
Microsoft Excel 2010 and laterT.TESTSee
LibreOffice CalcTTESTSee
Google SheetsTTESTSee
Pythonscipy.stats.ttest_indSee
MatlabttestSee
MathematicaTTestSee
Rt.testSee
SASPROC TTESTSee
JavatTestSee
JuliaEqualVarianceTTestSee
Statattest data1 data2See

Citations