Rudolph Anderson was born in 1879 in Haina, Sweden. At the age of 13, he moved to Boston where he attended an English grammar school from which he graduated by the time he turned 17. Deciding that continuing his education in High School was pointless for him, he started working on various industrial jobs during the day while studying during the night. That continued for several years, until, at one of the rubber manufacturing companies, he got a job as a laboratory boy and assistant to a chemist.
During the turn of the century, he got a chief chemist position at the manufacturing pharmaceutical company in New Orleans. In 1906, he graduated from Tulane University and got his Ph.D. from Cornell University Medical College. In 1909, he came back to Sweden where he applied for doctoral program at University of Uppsala. The university declined his application, and Anderson discouragly went to the University of Berlin where he was accepted to Emil Fischer's laboratory. The laboratory was already full, and he was transported to Hermann Leuchs laboratory where he was solving the problem on the chemistryof color reaction of combination of brucine with nitric acid. By 1911, Anderson completed the work for his doctoral degree but soon realized that he ran out of money. Fortunately his friend, Donald Dexter Van Slyke, received a letter from his father asking for recommendations. His father was a chief chemist at Agricultural Experiment Station in Geneva, New York and was seeking for a chemist there. Because he was recommended by his friend, Anderson went to Geneva, and soon after it was assigned to study cows metabolism. Later on he convinced the director that it would be important to know more about phytic acid before starting to experiment on dairy cows.
interrupted his studies in Berlin, Germany and he needed to abandon it for a time being. Later on he returned to Geneva where he continued on his work on phytic acid but also expanded his research to include important fragrance oils that he found in animal urine. He spent several months working side-by-side with Graham Lusk at Cornell Medical College where he learned about animal calorimetry. During the war, he volunteered and later commissioned as a Captain for the Sanitary Corps in the Division of Food and Nutrition. In 1919, he was discharged from the army, but before returning to Geneva he completed the necessary requirements for his Ph.D. in Lusk's laboratory.