RhoG was first identified as a coding sequence upregulated in hamster lung fibroblasts upon stimulation with serum. Expression of RhoG in mammals is widespread and studies of its function have been carried out in fibroblasts, leukocytes, neuronalcells, endothelial cells and HeLa cells. RhoG belongs to the Rac subgroup and emerged as a consequence of retroposition in early vertebrates. RhoG shares a subset of common binding partners with Rac, Cdc42 and RhoU/V members but a major specificity is its inability to bind to CRIB domain proteins such as PAKs.
As with all small G proteins RhoG is able to signal to downstream effectors when bound to GTP and unable to signal when bound to GDP. Three classes of proteininteract with RhoG to regulate GTP/GDP loading. The first are known as Guanine nucleotide exchange factors and these facilitate the exchange of GDP for GTP so as to promote subsequent RhoG-mediated signalling. The second class are known as GTPase activating proteins and these promote hydrolysis of GTP to GDP thus terminating RhoG-mediated signalling. A third group, known as Guanine nucleotide dissociation inhibitors, inhibit dissociation of GDP and thus lock the G protein in its inactive state. GDIs can also sequester G proteins in the cytosol which also prevents their activation. The dynamic regulation of G protein signalling is necessarily complex and the 130 or more GEFs, GAPs and GDIs described thus far for the Rho family are considered to be the primary determinants of their spatiotemporal activity. There are a number of GEFs reported to interact with RhoG, although in some cases the physiological significance of these interactions has yet to be proven. Well characterised examples include the dual specificity GEF TRIO which is able to promote nucleotide exchange on RhoG and Rac and also on RhoA via a separate GEF domain. Activation of RhoG by TRIO has been shown to promote NGF-induced neurite outgrowth in PC12 cells and phagocytosis of apoptotic cells in C. elegans. Another GEF, known as SGEF, is thought to be RhoG-specific and has been reported to stimulate macropinocytosis in fibroblasts and apical cup assembly in endothelial cells. Other GEFs reported to interact with RhoG include Dbs, ECT2, VAV2 and VAV3. There have been very few interactions reported between RhoG and negative regulators of G protein function. Examples include IQGAP2 and RhoGDI3.
Signalling downstream of RhoG
Activated G proteins are able to couple to multiple downstream effectors and can therefore control a number of distinct signalling pathways. The extent to which RhoG regulates these pathways is poorly understood thus far, however, one specific pathway downstream of RhoG has received much attention and is therefore well characterised. This pathway involves RhoG-dependent activation of Rac via the DOCK -family of GEFs. This family is divided into four subfamilies and it is subfamilies A and B that are involved in the pathway described here. Dock180, the archetypal member of this family, is seen as an atypical GEF in that efficient GEF activity requires the presence of the DOCK-binding protein ELMO which binds RhoG at its N-terminus. The proposed model for RhoG-dependent Rac activation involves recruitment of the ELMO/Dock180 complex to activated RhoG at the plasma membrane and this relocalisation, together with an ELMO-dependent conformational change in Dock180, is sufficient to promote GTP-loading of Rac. RhoG-mediated Rac signalling has been shown to promote neurite outgrowth and cell migration in mammalian cells as well as phagocytosis of apoptotic cells in C. elegans. Other proteins known to bind RhoG in its GTP-bound state include the microtubule-associated proteinkinectin, Phospholipase D1 and the MAP Kinase activator MLK3.
