Rhizobiales The Rhizobiales are an order of Gram-negative Alphaproteobacteria . The rhizobia , which fix nitrogen and are symbiotic with plant roots , appear in several different families. The four families Bradyrhizobiaceae , Hyphomicrobiaceae , Phyllobacteriaceae , and Rhizobiaceae contain at least six genera of nitrogen-fixing, legume-nodulating, microsymbiotic bacteria. Examples are the genera Bradyrhizobium and Rhizobium . Species of the Methylocystaceae are methanotrophs; they use methanol or methane as their sole energy and carbon sources. Other important genera are the human pathogens Bartonella and Brucella , as well as Agrobacterium .Taxonomy Bartonellaceae Gieszczykiewicz 1939 emend. Brenner et al . 1993 Beijerinckiaceae Garrity et al . 2006 Bradyrhizobiaceae Garrity et al . 2006 Brucellaceae Breed et al . 1957 Cohaesibacteraceae Hwang and Cho 2008 emend. Gallego et al . 2010 Mabikibacteraceae Choi et al. 2017 Hyphomicrobiaceae Babudieri 1950 Methylobacteriaceae Garrity et al . 2006 Methylocystaceae Bowman 2006 Phyllobacteriaceae Mergaert and Swings 2006 Rhizobiaceae Conn 1938 Rhodobiaceae Garrity et al . 2006 Roseiarcaceae Kulichevskaya et al . 2014 Xanthobacteraceae Lee et al . 2005Provisional families These families have been proposed but not yet validly published according to the rules of the Bacteriological Code ."Aurantimonadaceae" Denner et al . 2003 "Devosiaceae" Yarza et al . 2014 "Kaistiaceae" Yarza et al . 2014 "Labriaceae" Beck et al . 2015 "Lutibaculaceae" Yarza et al . 2014 "Methyloligellaceae" Yarza et al . 2014 "Methylopilaceae" Beck et al . 2015 "Pleomorphomonadaceae" Yarza et al . 2014 "Rhodomicrobiaceae" Yarza et al . 2014 "Stappiaceae" Yarza et al . 2014 "Thermopetrobacteraceae" Yarza et al . 2014 The following genera belong to the Rhizobiales but have not been assigned to a family. Alsobacter Bao et al . 2014 Amorphus Zeevi Ben Yosef et al . 2008 Bauldia Yee et al . 2010 Methylobrevis Poroshina et al . 2015 Methyloceanibacter Takeuchi et al . 2014 Methyloligella Doronina et al . 2014 Vasilyevaea Yee et al . 2010Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature and National Center for Biotechnology Information and the phylogeny is based on whole-genome sequences .has been reported in at least three Rhizobiales species: Agrobacterium tumefaciens , Methylobacterium organophilum , and Bradyrhizobium japonicum . Natural genetic transformation is a sexual process involving DNA transfer from one bacterial cell to another through the intervening medium, and the integration of the donor sequence into the recipient genome by homologous recombination .
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