Phosphatic fossilization has occurred in unusual circumstances to preserve some extremely high-resolution microfossils in which careful preparation can even reveal preserved cellular structures. Such microscopic fossils are only visible under the scanning electron microscope.
Mechanism
Large quantities of phosphate are required, either from seawater or from the tissues of the decaying organism. In some cases microbes control the phosphatization, and the remains of the microbes that fed on the preserved tissue form the fossil. In other, the tissue itself is the source of phosphate and its phosphatized remains form the fossil. In the intermediate case the phosphatized tissue retains the impressions of the phosphatizing microbes.
Phosphatic preservation in Burgess Shale-type fossils
Soft-tissue fossils, such as those found in the Burgess Shale, are rare. In some cases their internal organs are replicated in phosphate. The phosphate mainly comes from the tissue itself, and may later be replaced by calcium carbonate. A low pH makes CaCO3 less likely to precipitate, clearing the way for phosphate to be laid down. This is facilitated by the absence of oxygen in the decaying tissue. Accordingly, phosphate is generally only preserved in enclosed spaces, such as a tightly-closed bivalve shell. Higher concentrations of phosphate in the sea water do not enhance phosphatization, as may seem natural; rather, it increases the rate at which the organism breaks up, perhaps because the mineral "fertilizes" the decay micro-organisms. Phosphatization can happen quickly: The chitinous structures that support bivalve gills can be replaced by calcium phosphate, with a little help from co-occurring bacteria, in just two to six days. The gill axes and musculature of bivalves can also be preserved in phosphate. The structures that most famously preserved in phosphate in the Burgess Shale are the midgut glands of Leanchoilia, perhaps on account of their central position and plausibly a low pH? Phosphatization can be microbially mediated, especially in decay-resistant groups such as arthropods; or substrate-dominated, where phosphate-rich tissue leads the mineralization process. Cephalopods fall somewhere between these two extremes.
Phosphate-only fossils
In phosphatic fossils, the preservation is so fine that even some cellular structure has been preserved. The phosphatic microfossils of the Doushantuo Formation, a fossil-rich lagerstätte of the Ediacaran period, about 590–565 Ma, display some of the most spectacular cellular-level preservation known from the geologic record. The fossils include what may be metazoan blastulas, possibly animal embryos at an early stage in cell division. The Doushantuo Formation presents a classic example of phosphatic fossilization: A refinement to viewing the internal structure of fossilized embryos uses specialized microscopic three-dimensional X-ray computed tomography, a kind of micro CAT scan.
