Neuregulin Neuregulins or neuroregulins are a family of four structurally related proteins that are part of the EGF family of proteins . These proteins have been shown to have diverse functions in the development of the nervous system and play multiple essential roles in vertebrate embryogenesis including: cardiac development , Schwann cell and oligodendrocyte differentiation, some aspects of neuronal development , as well as the formation of neuromuscular synapses . Included in the family are heregulin ; neu differentiation factor ; acetylcholine receptor synthesis stimulator; glial growth factor ; and sensory and motor-neuron derived factor . Multiple family members are generated by alternate splicing or by use of several cell type-specific transcription initiation sites. In general , they bind to and activate the erbB family of receptor tyrosine kinases , erbB3 , and erbB4 ), functioning both as heterodimers and homodimers.Neuregulin family members The neuregulin family includes: Neuregulin-1 , with numerous discovered isoforms stemming from alternative splicing: * Type I NRG1 ; alternative names: Heregulin, NEU differentiation factor , or acetylcholine receptor inducing activity * Type II NRG1 ; alternative name: Glial Growth Factor-2 ; * Type III NRG1 ; alternative name: Sensory and motor neuron-derived factor ; * Type IV NRG1 ; * Type V NRG1 ; * Type VI NRG1 ; Types IV-VI are proteins with 3 novel N-terminal domains identified in 2004. Neuregulin-2 ; Neuregulin-3 ; Neuregulin-4 ; In mammals, neuregulin family members are the products of 4 genes NRG1, NRG2 , NRG3 and NRG4 respectively. The transmembrane forms of neuregulin 1 are present within synaptic vesicles , including those containing glutamate. After exocytosis , NRG1 is in the presynaptic membrane , where the ectodomain of NRG1 may be cleaved off. The ectodomain then migrates across the synaptic cleft and binds to and activates a member of the EGF-receptor family on the postsynaptic membrane . This has been shown to increase the expression of certain glutamate-receptor subunits. NRG1 appears to signal for glutamate-receptor subunit expression, localization, and /or phosphorylation facilitating subsequent glutamate transmission. The NRG1 gene has been identified as a potential gene determining susceptibility to schizophrenia by a combination of genetic linkage and association approaches.ARIA ARIA plays a role in synapse development, influencing the upregulation of acetylcholine receptor genes beneath the endplate after mammalian motor neurons have made synaptic contact with muscle fibres , hence its name ARIA = A cetylcholine R eceptor I nducing A ctivity.Animal models A study done on mice in early 2009 has indicated that when neuregulin-1\ErbB signalling is disrupted, the dendritic spines of neurons grow but do not fully form. This produced no immediate noticeable changes to brain development , but in adults there was a reduction of dendritic spines on neurons. Glutamatergic signalling was markedly disrupted in the mice as a result of the experiment.In fish, birds, and earthworms NRG-1,2,3 have been found in fish and birds. mRNA similar to mammalian Pro-NRG2 precursor has been found in humus earthworm Lumbricidae.
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