In 1596, Najran appeared in Ottoman tax registers as being in the nahiya of Bani Miglad in the Qada Hawran. It had a population of 65 households and 25 bachelors, all Muslim. The villagers paid taxes on wheat, barley, summer crops, occasional revenues, goats and beehives; a total of 5,000 akçe. Najran was settled by 200 Druze immigrant families from Mount Lebanon in 1685. The village had been previously abandoned by the Arab tribe Muqri al-Wahsh, although it still contained Christians. According to historians Hanna Abu Rashid and Bouron, Najran was the first place for the second wave of Mount Lebanon Druze to settle, while historian Sa'id Sghayar notes that two other villages in the Lejah plain were temporarily settled by this group of immigrants before Najran was chosen as a permanent residence. Historian Abu Shaqra described the Druze arrival at Najran:
As was the custom in such cases, the sheikh of Najran ordered the lighting of bonfires on the summit of the village... then bonfires were lit as a signal of war on the summit of every village in Jabel Hawran... In the morning came to Najran from every village. The three thousand Lebanese were distributed over the different parts of Hawran.
In 1711 Najran, which had a castle, became the center of the al-Hamdan clan, who controlled five other villages in the Hauran. In 1838, Edward Robinson was informed that Najran was a Catholic village, situated "in the Luhf, south of the Lejah". In the early 19th century Christians were still the majority in Najran, with roughly 150 families. There were 50 Druze families. By the mid-19th century the Druze Abu Fakhr clan controlled Najran and two other villages. During this period, the Christian and Druze communities were roughly equal in population and the chief of the village was Qasem Abu Fakhr. Traveler Josias Leslie Porter visited in the 1850s and noted that Najran had "extensive ruins... estimated at nearly two miles in circumference." The most significant of these was the Byzantine-era church, the remains of which consisted of two square-shaped towers with Greek inscriptions. One tower inscription contained the date 458 AD, while the other contained 564 AD. According to Porter, the ruins had functioned as a mosque in earlier times. By 1862 the Abu Fakhr chief was Ibrahim Abu Fakhr, who resided in Najran. In October 1895 the Ottoman army based in nearby al-Shaykh Maskin launched an offensive against the Druze, attacking Najran along with Qarrasa and Ahira. About 45 Ottoman soldiers were killed and 65 were wounded as a result of resistance by the three villages.