Masters W35 400 metres world record progression
This is the progression of world record improvements of the 400 metres W35 division of Masters athletics.
;Key:
Hand | Auto | Athlete | Nationality | Birthdate | Location | Date |
| 50.14 | Novlene Williams-Mills | | 26.04.1982 | Kingston | 25.06.2017 |
| 50.27 | Jearl Miles Clark | | 04.09.1966 | Madrid | 20.09.2002 |
| 50.37 | Jearl Miles Clark | | 04.09.1966 | Monaco | 19.07.2002 |
| 50.45 | Jearl Miles Clark | | 04.09.1966 | Rethymno | 07.07.2002 |
| 50.45 | Jearl Miles Clark | | 04.09.1966 | Palo Alto | 08.06.2002 |
| 50.56 A | Aurelia Penton | | 18.02.1943 | Medellin | 15.07.1978 |