List of winners of the Scottish League One and predecessors
A national third tier of Scottish league football was first established in the 1923–24 season as Division Three, but it only lasted for two full seasons due to financial difficulties.
As a result, a third-tier league was not competed until 1946–47 with the C Division, which also included reserve teams of the clubs from the divisions above. Division C operated as a national competition for the first three seasons; thereafter, it was split into two regional sections until season 1954–55, when the third tier was again abolished.
The third tier was re-established in 1975–76 season, when a division known as the Second Division was set up. Its status within the Scottish football league system league system changed in season 1998–99, when clubs from the top tier broke away from the Scottish Football League to form the Scottish Premier League. The Second Division was still the third tier of the Scottish league system, but became the second level of the Scottish Football League rather than the third. In 2013, the Scottish Premier League and Scottish Football League merged to form the Scottish Professional Football League, with the third tier becoming known as the Scottish League One.
Scottish Football League Division Three (1923–1926)
Scottish Football League Division C (1946–1955)
Scottish Football League Second Division (1975–2013)
Scottish League One (2013–)
Total wins
34 different clubs have won the third tier of Scottish football. Three clubs have won the third tier with both their first team and their reserve team.- Clubs participating in the 2019-20 Scottish League One are denoted in bold type.
- Clubs no longer active are denoted in italics.
| Club | Winners | Runners-up |
| Stirling Albion | 5 | 1 |
| Brechin City | 4 | 2 |
| Livingston | 4 | 1 |
| Clyde | 4 | — |
| Raith Rovers | 3 | 4 |
| Ayr United | 3 | 3 |
| Rangers 'A' | 3 | 2 |
| Greenock Morton | 3 | 1 |
| Queen of the South | 2 | 3 |
| Aberdeen 'A' | 2 | 2 |
| Dunfermline Athletic | 2 | 2 |
| Stranraer | 2 | 2 |
| Forfar Athletic | 2 | 1 |
| Clyde 'A' | 2 | — |
| Arbroath | 1 | 2 |
| East Stirlingshire | 1 | 2 |
| Heart of Midlothian 'A' | 1 | 2 |
| Hibernian 'A' | 1 | 2 |
| Partick Thistle 'A' | 1 | 2 |
| Airdrieonians | 1 | 1 |
| Berwick Rangers | 1 | 1 |
| Clydebank | 1 | 1 |
| Cowdenbeath | 1 | 1 |
| Dumbarton | 1 | 1 |
| Dundee 'A' | 1 | 1 |
| Falkirk | 1 | 1 |
| Montrose | 1 | 1 |
| Albion Rovers | 1 | — |
| Arthurlie | 1 | — |
| Gretna | 1 | — |
| Nithsdale Wanderers | 1 | — |
| Partick Thistle | 1 | — |
| Queen's Park | 1 | — |
| Rangers | 1 | — |
| Ross County | 1 | — |
| Alloa Athletic | — | 9 |
| East Fife | — | 2 |
| Hamilton Academical | — | 2 |
| Ayr United 'A' | — | 1 |
| East Fife 'A' | — | 1 |
| Inverness Caledonian Thistle | — | 1 |
| Kilmarnock | — | 1 |
| Leith Athletic | — | 1 |
| Morton 'A' | — | 1 |
| St Johnstone | — | 1 |