List of buildings in the Johnson Space Center
The buildings in the Johnson Space Center house facilities of the National Aeronautics and Space Administration's human spaceflight activities. The center consists of a complex of 100 buildings constructed on located in southeast Houston, Texas.
A typical building at Johnson Space Center is numbered and not named. A partial listing of building numbers and what is contained in them follows:
| Building | Description | Opened |
| 1 | Headquarters of JSC, including the offices of senior management and the JSC director | 1963 |
| 2 | Public Affairs Office, Media briefing room, video production, and audio processing facilities. | 1963 |
| 3 | Main cafeteria and JSC Exchange Store | 1963 |
| 4-N | Mission Operations support offices including Flight Director's Office | 1963 |
| 4-S | Mission Operations support offices and Flight Crew Operations Division | |
| 5 | Space Mission Simulation Facility | 1966 |
| 7 | Crew and Thermal Systems Division, including the vacuum chambers and space suit testing facilities, including the Environmental Test Article Shuttle airlock vacuum chamber and the Space Station Airlock Test Article vacuum chamber. | 1963 |
| 8 | Photographic Laboratory and Multimedia Operations Facility | 1963 |
| 9 | Space Vehicle Mockup Facility, including full-scale International Space Station module mockups and MPCV Orion developmental mockups. The Space Shuttle training mockups have been donated to outside organizations at the conclusion of the Space Shuttle Program, and have been removed. | 1966/1967 |
| 10 | Engineering fabrication facility and machine shop | 1963 |
| 11 | Satellite cafeteria and JSC Exchange Store | 1966 |
| 12 | Office of Education, Human Resources Office, Language Lab, and Financial Management Division | 1963 |
| 13 | Structures and Mechanics Laboratory | 1963 |
| 14 | Electromagnetic compliance studies building | 1966 |
| 15 | Human and Environmental Factors offices | 1966? |
| 16 and 16A | Shuttle Avionics Integration Laboratory, where software and hardware changes are tested to insure they function well with the whole vehicle in a simulated flight environment. Also houses the Shuttle Engineering Simulator. | 1963 |
| 17 | Space Food Systems Laboratory. Offices for Space Exploration. | |
| 18 | Morpheus Command Center | |
| 20 | Safety and Mission Assurance, Office of Procurement | |
| 21 | Human Health and Performance Laboratory | 2017 |
| 26 | Columbia Center | 2010 |
| 29 | Crew Exploration Vehicle Avionics Integration Laboratory; will be used to perform integrated avionics and flight software requirements verification. Originally served as the Weightless Environment Training Facility before the larger Neutral Buoyancy Laboratory was constructed to the north of the Johnson Space Center. The round portion of the building used to house a centrifuge which was used for astronaut training during the Gemini and Apollo eras. | 1966 |
| 30-A | Mission Operations Directorate functional offices The A in the building designation stands for Admin. | 1965 |
| 30-M | Christopher C. Kraft Jr. Mission Control Center - the historic Mission Control Center. This building is home to FCR1 which is the flight control room for International Space Station, FCR2 which is known as the Historic Apollo Control Room, as well as multiple controller consoles and the training flight control room. The M in the building designation stands for Main building. | 1965 |
| 30-S | Mission Control Center Space Station White FCR, Blue FCR, as well as flight consoles and data facilities. The S in the building designation stands for Space Station as the entire building, especially the White Flight Control Room, was originally built-out for use by Space Station Freedom. During the build-out decisions were made to share resources between the Space Shuttle, station, and the White FCR, was changed to Shuttle during this process. | 1993? |
| 31 | Astromaterials Research and Exploration Science | 1966 |
| 31-N | Lunar Sample Laboratory Facility | |
| 32 | Space Environment Simulation Laboratory; two very large thermal-vacuum chambers for testing flight hardware, designated as a National Historic Landmark | 1966 |
| 33 | Space Environment Simulation Test Facility with several small thermal-vacuum chambers for testing flight hardware | 1966 |
| 35 | Guidance and Navigation Simulation Facility | - |
| 37 | Life Sciences Laboratory; formerly the Lunar Receiving Laboratory | |
| 41 | Plant Operations Administration; formerly the Hypo/Hyperbaric Training Facility and Physiological Training Facility. Demolished on December 18, 2013. | 1970 |
| 44 | Communications and Tracking Division; formerly the Communications and Tracking Development Laboratory, Electronics Systems Test Laboratory and Audio Development Laboratory | 1966 |
| 45 | Project Engineering; also housed the Mission Evaluation Room | 1966 |
| 45-N | Occupational Clinic; formerly the Technical Library | 1966 |
| 48 | Mission Control Power Plant | |
| 49 | Vibration and Acoustic Test Facility | |
| 110 | Security headquarters just outside the NASA gates by the employee entrance. Security issues badges for employees, contractors, and visitors. | |
| 111 | Industry Assistance Office | |
| 222 | Atmospheric Reentry Materials and Structures Evaluation Facility; demolished in 2017. | 1966 |
| 225 | Administrative Support Facility Annex; formerly the Space Science Interim Facility; demolished on August 14, 2014. | 1968 |
| 226 | Administrative Support Facility Annex; first building constructed at JSC. Demolished on November 14, 2014. | 1963 |
| 259 | Astronaut Selection and Isolation Quarters | 1967 |
| 260 | Water Immersion Facility | |
| 263 | Health Physics Laboratory | |
| 268A | Planetary Analog Test Site, also known as the "Rock Yard". Simulates features of the lunar and Martian surface terrain, used for testing vehicle and spacesuit design |