The park includes areas of land which are part of Ku-ring-gai, Ryde, and Hornsbylocal government areas with small areas of the park in Willoughby, Lane Cove and Hunter's Hilllocal government areas on the banks of the lower reaches of the river. The park is surrounded on all sides by developed suburban areas and except for the upper northwestern region is never more than a kilometre wide. Much of the park is of fairly rugged terrain on the slopes of the river valley and covered by dense bush. The characteristics of the bush vary depending upon soils and topography. Parts of the park are affected by weed infestations, such as Fiddens Wharf and Fairyland. The central section of the park, between De Burghs Bridge on Ryde Road and Fullers Bridge, was set aside as a park in the 1920s and developed with picnic areas interspersed in the bush along the banks of the river. The majority of these picnic areas are located between the Fuller's Road bridge and the road that leads towards the Tourist Park. North of this point the area becomes more rugged and there are no picnic areas until the Tunks Hill area which is away from the river behind the garden nursery on Lane Cove Road. A weir was constructed in the 1930s near Fullers Bridge which converted the middle section of the river from tidal salt-water to a fresh-water area with constant water level suitable for recreational use in rowing boats and canoes. This area was managed by a trust and was called Lane Cove River Park. In 1982, the Government of New South Wales took over direct control of the park which was then called Lane Cove Regional Park. Various nearby areas of undeveloped government land near the river upstream and downstream from the central area including an area around Pennant Hills Park has also been added to the national park. It was then renamed Lane Cove National Park in 1992. A fish ladder has been constructed next to the weir to enable Australian bass to access the freshwater breeding grounds upstream of the weir. Fishing is allowed in parts of the Lane Cove River as per restrictions managed by NSW Department of Primary Industries. The Lane Cove National Park is popular with walkers, joggers and cyclists. The Lane Cove River Tourist Park, operated by the NSW National Parks & Wildlife Service, is located within Lane Cove National Park. The main walking track along the Lane Cove River forms part of the Great North Walk from Sydney CBD to Newcastle. This track incorporates a number of old tracks that were, in many cases, old logging trails.
The parks position in the middle of a major metropolis necessitates compromises between the social desire for maintaining a pristine environment, and the need for urban development. Two examples of this compromise are:
The M2 motorway crosses the National Park at Epping and is clearly visible for kilometers to bushwalkers traveling along Terry's Creek.
In 2014 Sydney Water began rebuilding a 3.3 kilometre section of wastewater pipe located beside Terrys Creek, and running through bushland between Forrester Park, Eastwood and Browns Waterhole in the Lane Cove National Park, South Turramurra. This involved upgrading access tracks to the pipeline.