ISAS is an objective tool for analyzing ictal vs. interictal SPECT scans. The goal of ictal SPECT is to localize the region of seizureonset for epilepsy surgery planning. ISAS was introduced and validated in two recent studies. This site is a technical supplement to, which should enable ISAS to be implemented at any center for further study and analysis.
Analysis
The basic idea of ISAS is to compute the difference between an ictal and interictal SPECT scan for a single patient. The differences of the ictal/inter-ictal comparison are checked against a healthy normal database to determine the normal expected variation. Significant increases and decreases in CBF between the interictal and ictal SPECT can then be detected. The analysis is conducted using SPM. Cerebral blood flow is known to increase during seizures at the site of seizure onset. Since SPECT is an indicator of CBF, increases in the SPECT during seizures can be useful for seizure localization. CBF decreases are more complicated, and occur both during and following seizures in multiple locations. Details of ISAS interpretation can be found in McNallyet al., 2005, but in summary: 1. For true ictal SPECT, seizure onset can be reliably localized based on SPECT increases. 2. For post-ictal SPECT, seizure onset cannot be reliably localized to a single lobe based on SPECT increase or decreases. However, the side of seizure onset can be reliably determined based on which hemisphere has greater overall SPECT decreases. The requirements for implementing ISAS are relatively simple. All that is needed is a computer running MATLAB and an operator with sufficient imaging experience to download and implement the SPM analysis. NOTE: ISAS has been tested and confirmed to work with SPM2 and the following MATLAB versions - 6.1, 6.5.1, and 7.0.4. In order to ensure correctness, both Matlab and SPM should be kept updated with the latest service packs and patches. Refer tothe software setup section of our site for more information. The intent of ISAS and the ISAS website is to begin to fill the need for epilepsy SPECT image analysis, by providing a freely available method that can be implemented anywhere.