High Five cells
High Five is an insect cell line that originated from the ovarian cells of the cabbage looper, Trichoplusia ni. It was developed by the Boyce Thompson Institute for Plant Research, Ithaca, NY.
High Five cells have become one of the most commonly used cell lines for recombinant protein expression using baculovirus or transfection, and have been demonstrated, in a number of cases, to express more recombinant protein than other lepidopteran cell lines, such as Sf9 cells. They can be grown in the absence of serum, and can be cultured in a loose attached state or in suspension. Hi5 cells produce abundant microRNAs, small interfering RNAs, and PIWI-interacting RNAs, making it suitable to study all three types of small silencing RNAs.
