A high-frequency recombination cell is a bacterium with a conjugativeplasmid integrated into its chromosomal DNA. The integration of the plasmid into the cell's chromosome is through homologous recombination. A conjugative plasmid capable of chromosome integration is also called an episome. When conjugation occurs, Hfr cells are very efficient in delivering chromosomal genes of the cell into recipient F− cells, which lack the episome.
An Hfr cell can transfer a portion of the bacterial genome. Despite being integrated into the chromosomal DNA of the bacteria, the F factor of Hfr cells can still initiate conjugative transfer, without being excised from the bacterial chromosome first. Due to the F factor's inherent tendency to transfer itself during conjugation, the rest of the bacterial genome is dragged along with it. Therefore, unlike a normal F+ cell, Hfr strains will attempt to transfer their entire DNA through the mating bridge, in a fashion similar to the normal conjugation. In a typical conjugation, the recipient cell also becomes F+ after conjugation as it receives an entire copy of the F factor plasmid; but this is not the case in conjugation mediated by Hfr cells. Due to the large size of bacterial chromosome, it is very rare for the entire chromosome to be transferred into the F − cell as time required is simply too long for the cells to maintain their physical contact. Therefore, as the conjugative transfer is not complete, the recipient F− cells do not receive the complete F factor sequence, and do not become F + due to its inability to form a sex pilus.
In conjugation mediated by Hfr cells, transfer of DNA starts at the origin of transfer located within the F factor and then continues clockwise or counterclockwise depending on the orientation of F factor in the chromosome. Therefore, the length of chromosomal DNA transferred into the F− cell is proportional to the time that conjugation is allowed to happen. This results in sequentialtransfer of genes on the bacterial chromosome. Bacterial geneticists make use of this principle to map the genes on the bacterial chromosome. This technique is called interrupted mating as geneticists allow conjugation to take place for different periods of time before stopping conjugation with a high-speed blender. By using Hfr and F− strains with one strain carrying mutations in several genes, each affecting a metabolic function or causing antibiotic resistance, and examining the phenotype of the recipient cells on selective agar plates, one can deduce which genes are transferred into the recipient cells first and therefore are closer to the oriT sequence on the chromosome.
The F-prime cell
Is a form of cell Comes from Hfr chromosome crossing, that a portion of plasmid and a parts of chromosomal DNA distinct about whole Hfr genome. For result, the new cell contains plasmid and pure chromosome. The new plasmid contains a parts of chromosomal DNA and the segment of a plasmid that was in Hfr genome.
