were slightly larger than the original Type IXCs. U-541 had a displacement of when at the surface and while submerged. The U-boat had a total length of, a pressure hull length of, a beam of, a height of, and a draught of. The submarine was powered by two MAN M 9 V 40/46 supercharged four-stroke, nine-cylinder diesel engines producing a total of for use while surfaced, two Siemens-Schuckert 2 GU 345/34 double-acting electric motors producing a total of for use while submerged. She had two shafts and two propellers. The boat was capable of operating at depths of up to. The submarine had a maximum surface speed of and a maximum submerged speed of. When submerged, the boat could operate for at ; when surfaced, she could travel at. U-541 was fitted with six torpedo tubes, 22 torpedoes, one SK C/32 naval gun, 180 rounds, and a SK C/30 as well as a C/30 anti-aircraft gun. The boat had a complement of forty-eight.
For her second foray, U-541 headed toward the eastern seaboard of North America. On her third sortie, she sank the Livingston northeast of Louisbourg, Nova Scotia. The boat was preparing to attack a convoy while on the surface in the Gulf of St. Lawrence when opened fire; U-541 was forced to dive. She was then hunted for two days by four frigates, a minesweeper and aircraft of the Royal Canadian Air Force, but escaped. On 26 May 1944, on its way from Lisbon to Port Richmond, Philadelphia, USA, the Serpa Pinto was stopped in the mid-Atlantic by the U-541. The U-boat's captain ordered the Serpa Pinto's crew and passengers to abandon the ship in the lifeboats, and requested permission from Kriegsmarine headquarters to torpedo the ship. The passengers and crew, with the exception of the captain who decided to remain on board whatever the German decision, duly left the ship in the lifeboats. There they were forced to wait all night while the German U-boat awaited a reply to its request. By dawn an answer had arrived from Admiral Karl Dönitz, who refused permission to sink the ship. The U-boat then departed the area and the lifeboats returned to the ship. The ship's doctor, a cooker and a 15 months child drowned during this incident. Two military-aged Americans were taken in the submarine.
4th patrol
Her last patrol began in Horten Naval Base in Norway on 7 April 1945. It ended with her surrender in Gibraltar on 12 May 1945.
Fate
U-541 was transferred to Lisahally in Northern Ireland for Operation Deadlight. She was sunk on 5 January 1946 at.