GRASP65


Golgi reassembly-stacking protein of 65 kDa also known as Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene.

Function

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The GRASP65 protein is a peripheral membrane protein anchored to the lipid bilayer through myristoylation of a glycine residue near the protein's amino terminus. It is involved in establishing the stacked structure of the Golgi apparatus and linking the stacks into larger ribbons in vertebrate cells. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. GRASP65 can form a complex with the Golgi matrix protein GM130, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined.

Structure

GRASP65 contains two PDZ domains in the amino-terminal GRASP domain, that comprises approximately half of the protein. The GRASP region interacts with the Golgi matrix protein GM130 as well as an intrinsically disordered region in the C-terminus.

Interactions

GORASP1 has been shown to interact with TGF alpha, TMED2 and GOLGA2.