Inspired to visit Africa by the diaries of David Livingstone, Holub travelled to Cape Town, South Africa shortly after graduation and eventually settled in Dutoitspan near Kimberley to practise medicine. After eight months, Holub set out in a convoy of local hunters on a two-month experimental expedition, or "scientific safari", where he began to assemble a large natural history collection. in 1870s In 1873, Holub set out on his second scientific safari, devoting his attention to the collection of ethnographic material. On his third expedition in 1875, he ventured all the way to the Zambezi river and made the first detailed map of the region surrounding Victoria Falls. Holub also wrote and published the first book account of the Victoria Falls published in English in Grahamstown in 1879. After returning to Prague for several years, Holub made plans for a bold African expedition. In 1883, Holub, along with his new wife Rosa and six European guides, set out to do what no one had done before: explore the entire length of Africa from Cape Town all the way to Egypt. However, the expedition was troubled by illness and the uncooperative Ila tribesmen and Holub's team was forced to turn back in 1886. Holub mounted two exhibitions, highly attended but ending up in financial loss, in 1891 in Vienna and in 1892 in Prague. Frustrated that he was unable to find a permanent home for his large collection of artefacts, he gradually sold or gave away parts of it to museums, scientific institutions and schools. Later Holub published a series of documents, contributing to papers and magazines, and delivering lectures. His early death came in Vienna on 21 February 1902, from lingering complications of malaria and other diseases he had acquired while in Africa.
Commemorations of Holub's legacy
In 1949, a monument to Holub by Jindřich Soukup was unveiled in his hometown of Holice.
In 1952, Czech movie Velké dobrodružství was filmed about Holub's expeditions.
In 1970, the town of Holice opened a museum dedicated to Emil Holub near the main post office with an associated monument nearby.
In September 2005, exactly 130 years since Holub's first visit to the Victoria Falls, a bust of Holub by the Zimbabwean sculptor Last Mahwahwa was unveiled by the Ambassador of the Czech Republic, Jaroslav Olša, jr., and the Ambassador of the Republic of Austria, Michael Brunner, in front of the National Museum of Zambia in Livingstone, the city adjacent to the Victoria Falls.
Works
In German
Gabriele Riz: Leben und Werk des Afrikaforschers Emil Holub. 1847–1902. Diplomarbeit. Universität Wien, Wien 1985.
In English
Dark Deeds. Some Hunting Memoirs of the Nineteenth Century Czech Traveller Emil Holub