Casamino acid
Casamino acids is a mixture of amino acids and some very small peptides obtained from acid hydrolysis of casein. It is typically used in microbial growth media. It has all the essential amino acids except tryptophan, which is destroyed by digestion with sulfuric or hydrochloric acid.
Casamino acids is similar to tryptone, the latter differing by being an incomplete enzymatic hydrolysis with some oligopeptides present, while casamino acids is predominantly free amino acids.Uses
Casamino acids supplies a completely hydrolyzed protein nitrogen source. It contains a small amount of cystine. Tryptophan and vitamins are destroyed by the acid treatment. The remaining amino acids are a source of nutrients for various microorganisms. Amino acids are highly soluble and suitable for use in tissue culture. Salt content is typically 30-40%. Casamino acids are either found in the Daptacel brand DTaP vaccine or used in its manufacture.Appearance
