The impetus for Hampton's use of gloves was her sensitive skin. Previously she had to wear gloves when gardening, and the procedures at the hospital caused her to develop severe contact dermatitis and painful eczema. Halsted, as a supporter of the germ theory of disease, used strict hygienic measures in his operating theater. People had to cleanse their hands using soap, followed by a caustic solution of potassium permanganate, a hot oxalic acid bath, and a mercury chloride compound. In the winter of 1889 Caroline Hampton informed Halsted of her intention to resign due to the effects on her hands. Halsted considered Hampton an “unusually efficient” operating room assistant. He suggested that she try coating her hands in a gelatinous substance called collodion. It hardened but tended to crack. Next Halsted sent plaster casts of Caroline’s hands to the Goodyear Rubber Company of New York, and ordered two pairs of "bespoke" rubber gloves to cover her hands and forearms. The resulting gloves were thin, flexible, and reusable, and protected Hampton's hands. Halsted was surprised to return from an extended vacation and find that others in the operating room had also adopted rubber gloves, following Caroline's example. The gloves protected the medical staff's hands, and surgical assistants reported that the textured surface of the gloves made it easier to hold onto slippery surgical instruments. That they also protected patients was discovered by one of Halsted’s surgical residents, Joseph Colt Bloodgood. In 1899, Bloodgood published results showing that use of gloves during surgery reduced postsurgical infection rates from 17% to less than 2%, a staggering effect.
Marriage
Caroline Hampton and William Halsted were married on June 4, 1890, at Trinity Episcopal Church, Columbia, South Carolina. Caroline, as a married woman, was required to resign from her job at the hospital. Caroline ran their household during the winter, and their farm High Hampton in North Carolina during the summer. Caroline suffered later in life from migraines, and it is suspected that she, as well as her husband, may have used morphine. Both were known for their eccentricity. Anecdotes about their modes of entertaining, attachment to pets and marital life amused local society.