Carboxypeptidase


A carboxypeptidase is a protease enzyme that hydrolyzes a peptide bond at the carboxy-terminal end of a protein or peptide. This is in contrast to an aminopeptidases, which cleave peptide bonds at the N-terminus of proteins. Humans, animals, bacteria and plants contain several types of carboxypeptidases that have diverse functions ranging from catabolism to protein maturation.

Mechanism

The mechanism to produce carboxypeptidase involve that the substrate coordinate water is replaced by substrate of carbonyl groups.

Functions

The first carboxypeptidases studied were those involved in the digestion of food. However, most of the known carboxypeptidases are not involved in catabolism; they help to mature proteins or regulate biological processes. For example, the biosynthesis of neuroendocrine peptides such as insulin requires a carboxypeptidase. Carboxypeptidases also function in blood clotting, growth factor production, wound healing, reproduction, and many other processes.

Classifications

By active site mechanism

Carboxypeptidases are usually classified into one of several families based on their active site mechanism.
These names do not refer to the selectivity of the amino acid that is cleaved.

By substrate preference

Another classification system for carboxypeptidases refers to their substrate preference.
A metallo-carboxypeptidase that cleaves a C-terminal glutamate from the peptide N-acetyl-L-aspartyl-L-glutamate is called "glutamate carboxypeptidase".
A serine carboxypeptidase that cleaves the C-terminal residue from peptides containing the sequence -Pro-Xaa is called "prolyl carboxypeptidase".

Activation

Some, but not all, carboxypeptidases are initially produced in an inactive form; this precursor form is referred to as a procarboxypeptidase. In the case of pancreatic carboxypeptidase A, the inactive zymogen form - pro-carboxypeptidase A - is converted to its active form - carboxypeptidase A - by the enzyme trypsin. This mechanism ensures that the cells wherein pro-carboxypeptidase A is produced are not themselves digested.