Cannabidiolic acid synthase
Cannabidiolic acid synthase is an enzyme with systematic name cannabigerolate:oxygen oxidoreductase . It is an oxidoreductase found in Cannabis sativa that catalyses the formation of cannabidiolate, a carboxylated precursor of cannabidiol.Cannabidiolic acid synthase consists of a single protein with a molecular mass of 74 kDa. Its amino acid sequence is partly homologous to several other oxidoreductases, such as berberine bridge enzyme in Eschscholzia californica and Nectarin V in Nicotiana langsdorffii X N. sanderae.
CBDA synthase has four binding sites; two for FAD and two for the substrate.Enzyme function
Cannabidiolic acid synthase catalyses the production of cannabidiolate predominantly from cannabigerolate by stereospecific oxidative cyclization of the geranyl group of cannabigerolic acid according to the following chemical reaction:
Cannabinerolate can also be used as a substrate, but with lower efficiency than cannabigerolate. It covalently binds FAD, and does require coenzymes & molecular oxygen for the oxidocyclization reaction.
The optimum pH for CBDA synthase is 5.0.
