Rein was born and raised in Niles, Ohio, where he is still remembered as a legendary high school athlete for the Red Dragons of Niles McKinley High School. Rein played at Niles during their heyday, when the Red Dragons under coach Tony Mason were one of the top big school powerhouses in high school football in Ohio.
Baseball career
Rein played baseball at Ohio State University from 1965 through 1967, helping the Buckeyes win the 1966 College World Series, the school's only NCAA baseball title. Rein played shortstop and left field. He led his team in stolen bases in 1965 and 1966, and in doubles and runs in 1966. Rein had 49 career stolen bases, which stood as a team record until he was surpassed by Roy Marsh in the early 1990s. In 1965 and 1966, Ohio State participated in the College World Series, and Rein was selected in both years to the All Tournament team. In 1965, the Buckeyes lost the championship game to Arizona State. In 1966, Ohio State won the championship, defeating Oklahoma State. In the championship game, Rein contributed a double. After he finished his college career, Rein was drafted by the Cleveland Indians. He was playing for the Portland Beavers, the Indians' Triple-Afarm team, when Achilles tendon and hamstring problems ended his baseball career.
Football playing career
From 1964 to 1966, Rein was a three-year starter at left halfback for the Ohio State Buckeyes football team. He led his team in receptions in 1964 and 1965, and in rushing in 1966. Rein finished at Ohio State the team career receptions leader. Following his Ohio State career, Rein was drafted by the Baltimore Colts. Former teammate, and later Mayor of Columbus, Ohio, Greg Lashutka said of Rein, "He wasn't the biggest guy, but pound for pound he was tough as they come. He had that inner drive and did everything to the fullest. He could play." A continuing tradition at Ohio State is that at the end of every season, the team votes to award one teammate the "Bo Rein Most Inspirational Player Award."
Following the 1979 season, Rein was hired away from NC State by Louisiana State University. In January 1980, Rein took a recruiting trip to Shreveport, Louisiana. On his January 10, 1980 return trip back to Baton Rouge, Louisiana, his private aircraft crashed, leaving no survivors. Rein and experienced pilot Louis Benscotter left Shreveport in a Cessna 441 aircraft. The flight was planned to be a 40-minute trip, but when Benscotter rerouted east to avoid a storm, air traffic control lost contact with him. The aircraft climbed to 40,000 feet and kept heading due east. After being tracked on radar, the aircraft was eventually intercepted by U.S. Air National GuardConvair F-106 Delta Dart fighter aircraft over North Carolina, a thousand miles off-course and at an altitude of, feet higher than its maximum certified ceiling. The military pilots could not see anyone in the cockpit and the aircraft continued on over the Atlantic Ocean, where it crashed after running out of fuel. A US Coast Guard crew spotted some debris, but no wreckage was ever recovered. The bodies of Rein and Benscotter have never been found. The cause of the crash is undetermined but was most likely cabin depressurization causing hypoxia, a lack of oxygen, resulting in the occupants losing consciousness. In 1982, Rein's widow, Suzanne Kay, reached an out-of-court settlement for an undisclosed amount after filing a $10 million damage suit against a number of defendants, including the Cessna Aircraft Company, Cruse Aviation Inc., who serviced the aircraft, and Nichols Construction Corporation, who owned it.