Athletics at the 2007 Pan American Games – Women's 400 metres
The women's 400 metres event at the 2007 Pan American Games was held on July 23–25.Medalists
Results
Heats
Qualification: First 2 of each heat and the next 2 fastest qualified for the final.
| Rank | Heat | Name | Nationality | Time | Notes |
| 1 | 1 | Ana Guevara | | 51.10 | Q |
| 2 | 2 | Davita Prendergast | | 51.47 | Q |
| 3 | 2 | Indira Terrero | | 51.56 | Q |
| 4 | 3 | Shereefa Lloyd | | 51.66 | Q |
| 5 | 3 | Christine Amertil | | 51.67 | Q, SB |
| 6 | 2 | Debbie Dunn | | 51.86 | q |
| 7 | 1 | LaVerne Jones | | 51.94 | Q, PB |
| 8 | 1 | Monique Henderson | | 52.18 | q |
| 9 | 3 | Gabriela Medina | | 52.22 | |
| 10 | 2 | Kineke Alexander | | 52.37 | |
| 11 | 1 | Maria Laura Almirão | | 52.72 | |
| 12 | 1 | Esther Akinsulie | | 52.77 | |
| 13 | 3 | Aliann Pompey | | 53.03 | |
| 14 | 2 | Ginou Etienne | | 53.13 | |
| 15 | 3 | Carline Muir | | 53.25 | |
| 16 | 3 | Nathandra John | | 53.42 | |
| 17 | 2 | María Idrobo | | 54.29 | |
| 18 | 3 | Sheila Ferreira | | 54.44 | |
| 19 | 3 | Britney St. Louis | | 54.45 | |
| 20 | 1 | Lucy Jaramillo | | 54.55 | |
| 21 | 2 | Hazel Ann Regis | | 54.75 | |
| 1 | Dominique Maloney | | | |
Final
