Anne was the daughter of Sir Philip Calthorpe of Burnham Thorpe, Norfolk and his second wife, Jane Blennerhassett. Anne had an older half-brother Sir Philip Calthorpe who married Jane Boleyn, paternal aunt of Queen Anne Boleyn.
Anne had two stepsons, Thomas and Henry from her husband's first marriage. On 27 November 1542, her husband succeeded as the 2nd Earl of Sussex, and from that date onward, she was styled Countess of Sussex. He also became the 11th Baron of FitzWalter, and the 2nd Viscount FitzWalter. In 1543, she went to court as a lady-in-waiting to the Queen, whose Reformed beliefs she shared. She was one of the Queen's ladies personally named by Anne Askew's interrogators. She was questioned by a commission for "errors in scripture". Her marriage to Radcliffe was not successful as they differed on religious issues. Between 1547 and 1549, they separated after he expelled her from their home, accusing her of having entered into a bigamous marriage with Sir Edmund Knyvet. In a letter written by Anne to her mother, she alleged that Radcliffe had thrown her out of his house without "money, men, women, or meat, and no more than two velvet gowns".
Imprisonment and divorce
In September 1552, Anne was sent to the Tower of London for having practised sorcery and having made "treasonous prophecies". She was released five and a half months later. Following the accession of Mary I to the English throne, Anne fled to the Continent to avoid persecution for her Protestant beliefs. In her absence, Radcliffe had a Bill introduced in Parliament against the "adulterous living of the late Countess of Sussex". It didn't pass. The following year, Radcliffe attempted to bastardise her children with another Parliamentary Bill, but this also failed to pass despite having been read three times in the House of Commons of England. In 1555, he tried again with yet another Bill, this time to prevent her from enjoying her dowry or jointure rights which did pass; however, he no longer sought to bastardise her children. He described Anne as having been "unnatural and unkind". They divorced on 13 November 1555.
Return to England
Shortly after Radcliffe's death in February 1557, she returned to England. In his will, Radcliffe styled her as an "unkind wife". By April of that same year she was a prisoner in the Fleet. The following year, a Bill of Parliament settled the matter of her jointure, and in 1559, she married secondly, Andrew Wyse, a former Royal Officer in Ireland. Their marriage is confirmed in the Patent Rolls of Chancery in Ireland. By her second husband, Anne bore two more children:
Elizabeth Wyse ; married Alexander Fitton on 31 October 1578.
Anthony Wyse
Last years and death
Anne, her husband and their children returned to Ireland in 1564. She died sometime between 22 August 1579 and 28 March 1582.