7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase


7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase and 5-amino-6-uracil—L-tyrosine 4-hydroxyphenyl transferase are two enzymes always complexed together to achieve synthesis of FO, a precursor to Coenzyme F420. Their systematic names are 5-amino-5--6--5,6-dihydrouracil ammonia-lyase and 5-amino-6-uracil:L-tyrosine, 4-hydroxyphenyl transferase respectively. The enzymes catalyse the following chemical reactions:
Enzyme 2.5.1.147 binds a 4Fe-4S cluster. The condensation reaction is initiated by the 5'-deoxyadenosyl radical. The complex was formerly named as a single entity under.
All members of these enzymes belong to a single clade of the CofH and CofG families, sharing a TIM barrel structure. The two EC numbers represent discrete steps in this reaction. Some enzyme complexes have CofG and CofH in different chains assembled into a heterodimer, while others like the bifunctional fbiC has the two domains on one single chain.