| Rank | Heat | Nation | Athletes | Time | Notes |
| 1 | 3 | | Matthew Boling, Nicholas Ramey, Justin Robinson, Umajesty Williams | 3:05.57 | Q, |
| 2 | 1 | | Alex Haydock-Wilson, Joseph Brier, Ellis Greatrex, Alex Knibbs | 3:07.17 | Q, |
| 3 | 2 | | Klaudio Gjetja, Andrea Romani, Lorenzo Benati, Edoardo Scotti | 3:08.35 | Q, |
| 4 | 2 | | Adam Kopp, Christian Davis, Harvey Murrant, Ashley Moloney | 3:08.53 | Q, |
| 5 | 2 | | Justus Ringel, Joscha Bretschneider, Arne Leppelsack, Jean Paul Bredau | 3:08.53 | q, |
| 6 | 3 | | Fabrisio Saïdy, Téo Andant, Antoine Leblois, Lidji Mbaye | 3:08.78 | Q, |
| 7 | 3 | | Pasindu Kodikara, Pabasara Niku, Thiran Ravishka, Aruna Dharshana | 3:08.88 | q |
| 8 | 1 | | Simon Mazebo, Sven Van Den Bergh, Rayane Borlée, Jonathan Sacoor | 3:09.39 | Q, |
| 9 | 3 | | Julien Bonvin, Ricky Petrucciani, Filippo Moggi, William Reais | 3:09.48 | |
| 10 | 3 | | Dickson Clever Kapandura, Ngoni Joel Chadyiwa, Blessing Nyandoro, Leon Tafirenyika | 3:09.68 | |
| 11 | 2 | | Akin Özyürek, Mahsum Korkmaz, Ilyas Canakci, Ahmet Kasap | 3:09.87 | |
| 12 | 1 | | Shuji Mori, Daichi Sawa, Hayata Goto, Yusuke Shirao | 3:10.06 | |
| 13 | 1 | | Jacek Majewski, Sebastian Jakubowski, Adam Mroczek, Szymon Kreft | 3:10.68 | |
| 14 | 3 | | Khamal Stewart-Baynes, Ruach Chuol Padhal, Nathaniel St. Romain, Myles Misener-Daley | 3:11.58 | |
| 15 | 1 | | Mosisa Siyoum, Melkamu Assefa, Gadisa Bayu, Abdurahman Abdo | 3:12.63 | |
| 16 | 2 | | Rajesh Ramesh, Devender Kumar, Stanley Cibbinkumar, Ayush Dabas | 3:14.19 | |
| 17 | 1 | | Ondrej Holub, Vladislav Sadirov, Ladislav Topfer, Tomáš Nemejc | 3:14.71 | |
| 18 | 2 | | Malesela Senona, Thembo Monareng, Lourens Steenekamp, Gabriel Louw | 3:26.44 | |
| 19 | 1 | | Malik Smith, Dashawn Morris, Anthony Carpenter, Shemar Chambers | | |
| Rank | Nation | Athletes | Time | Notes |
| | Klaudio Gjetja, Andrea Romani, Alessandro Sibilio, Edoardo Scotti | 3:04.05 | , |
| | Elija Godwin, Nicholas Ramey, Justin Robinson, Howard Fields | 3:05.26 | |
| | Alex Haydock-Wilson, Joseph Brier, Alastair Chalmers, Alex Knibbs | 3:05.64 | |
| 4 | | Fabrisio Saïdy, Téo Andant, Antoine Leblois, Lidji Mbaye | 3:06.65 | |
| 5 | | Sven Van den Bergh, Jonathan Sacoor, Simon Mazebo, Rayane Borlée | 3:07.05 | |
| 6 | | Justus Ringel, Joscha Bretschneider, Arne Leppelsack, Jean Paul Bredau | 3:07.80 | |
| 7 | | Adam Kopp, Christian Davis, Harvey Murrant, Ashley Moloney | 3:09.31 | |
| 8 | | Pasindu Kodikara, Pabasara Niku, Thiran Ravishka, Aruna Dharshana | 3:09.38 | |
