2016 Italian Open – Women's Singles
was the defending champion, but could not defend the title because of her provisional suspension after failing a drug test at the Australian Open.
Serena Williams won the title in Rome for the fourth time, defeating Madison Keys in the final, 7–6, 6–3. This was Williams's second Time title without dropping a set.Seeds
The top eight seeds received a bye into the second round.Draw
Finals
Top half
Section 2
Section 3
Section 4
Qualifying
Seeds
Qualifiers
Draw
Second qualifier
Third qualifier
Sixth qualifier