The 2016 Championship Game would be the sixth in the Big Ten's 121-year history, and the third to feature the conference's East and West division alignment. Last season, the Big Ten Championship Game featured the Michigan State Spartans, champions of the East Division, and the Iowa Hawkeyes, champions of the West Division. Iowa made its first appearance in the conference championship game, while Michigan State made its third appearance. Michigan State defeated Iowa 16–13 to win its second Big Ten Championship in three years.
The Badgers started the season unranked, and opened vs the No. 5 LSU Tigers, whom they upset, 16–14, at Lambeau Field. The Badgers remained ranked the remainder of the season, peaking at No. 5 in both the AP and Coaches polls, and at No. 6 in the CFP rankings released on November 22 and November 29, 2016. The Badgers went into the Championship game 10–2, with both losses coming to Top 5 teams: No. 4 Michigan, who defeated Wisconsin 14-7; and No. 2 Ohio State, who defeated Wisconsin 30-23 in overtime.
The Nittany Lions started the season off 2–2 after losses to Pitt and No. 4 Michigan but finished the regular season on an 8-game winning streak as well as beating No. 2 Ohio State while averaging 35 points per game. They clinched the Big Ten East and a spot in the Big Ten Championship with a win and a Michigan loss to Ohio State. The Nittany Lions were led by sophomore duo quarterbackTrace McSorley and 1,000+ yard rusher in Saquon Barkley and junior wide receiverChris Godwin. They finished the regular season 10–2, a big improvement from the last two seasons under head coachJames Franklin. Following Penn State's upset of No. 2 Ohio State, the Nittany Lions would be ranked No. 24 in the AP poll, their first appearance in the poll since December 4, 2011, where they were ranked No. 23 prior to their loss to No. 17 Houston in the TicketCity Bowl. The following week, following their 62-24 victory over Purdue, Penn State was ranked No. 23 in the Coaches poll, their first appearance in said poll since December 4, 2011. Furthermore, Penn State made its debut in the CFP's first ranking of the season on November 1, where they were placed at No. 12; throughout the remainder of the season, the Nittany Lions continued to climb, maxing out at No. 7/8 heading into the Big Ten Championship Game.