2000–01 Eerste Divisie
The Dutch Eerste Divisie in the 2000–01 season was contested by 18 teams. FC Den Bosch won the championship.New entrants
Relegated from the 1999–2000 Eredivisie
Promotion/relegation play-offs
In the promotion/relegation competition, eight entrants entered in two groups. The group winners were promoted to the Eredivisie.
